3D Microelectrodes on Chip for Impedance-Based Cell Detection and Differentiation

Rollo, Enrica ;   Wilhelm, Jannick ;   Guiducci, Carlotta

Tags MicrofabricationLab-on-ChipMicrofluidics

In this work we show single-particle differentiation in flow based on size and cell state by means of on-chip 50 µm high 3D silicon micropillar...

Read More Download this Paper

A Magnetic Microparticle Capture and Liposome-Based Impedance Sensing Device for Detection of Viruses in Blood

Damhorst, Gregory ;   Sobieraj, Magdalena ;   Chaves, Rodrigo ;   Kooiman, Jonathan ;   Smith, Cartney ;   Kong, Hyunjoon ;   Bashir, Rashid

Tags Global healthMicrofluidicsDiagnostics

Low-cost, rapid detection of viruses in blood at the point-of-care remains a challenge and an unmet global need in the management of a...

Read More Download this Paper

A Medium Throughput Device to Study the Effects of Combinations of Surface Strains and Fluid-Flow Shear Stresses on Cells

Sinha, Ravi ;   Le Gac, Séverine ;   Verdonschot, Nico ;   van den Berg, Albert ;   Koopman, Bart ;   Rouwkema, Jeroen

Tags Regenerative medicineLab-on-ChipMicrofluidics

We report a 96-well-plate-sized device to screen for the effects on cells of all combinations of five equibiaxial surface strains (2-20%...

Read More Download this Paper

A Microfluidic Device to Investigate the Effect of Alternating Electric Field on Cancer Cells

Pavesi, Andrea ;   Adriani, Giulia ;   Ping, Andy T.K ;   Warkiani, Majid E. ;   Kamm, Roger D.

Tags MicrofluidicsLab-on-Chip

We present a microfluidic device to investigate the effect of electric field on cancer cells proliferation and migration in a 3D matrix and in...

Read More Download this Paper

A Microfluidic Platform to Study and Modulate the Blood-Brain Barrier

van der Helm, Marieke Willemijn ;   Frimat, Jean-Philippe ;   Odijk, Mathieu ;   van den Berg, Albert ;   Segerink, Loes Irene

Tags Lab-on-ChipBioMEMSMicrofluidics

A simple yet predictive model of the blood-brain barrier (BBB) is required to help drug development and improve the understanding of the...

Read More Download this Paper

Advance Diagnostic and Sample Preparation Platform for Biosurveillance

Jebrail, Mais ;   Sinha, Anupma ;   Renzi, Ron ;   Bartsch, Micheal ;   Van De Vreugde, Jim ;   Branda, Steven ;   Patel, Kamlesh

Tags Global healthPoint-of-careMicrofluidics

We report on our work to develop a forward-deployed sample preparation and diagnostic platform to safely and consistently process infected...

Read More Download this Paper

An Engineered Microfluidic Device for Modeling Cardiac Tissues

Acun, Aylin ;   Zorlutuna, Pinar

Tags MicrofluidicsStem cellsTissue engineering

Studying the heart tissue is critical for understanding and developing treatments for cardiovascular diseases. In this work, we present a...

Read More Download this Paper

An Image Reconstruction Method Using Singular Value Decomposition for Magnetic Particle Imaging

Shimizu, Shota ;   Tsuchiya, Hiroki ;   takagi, tomoyuki ;   Hatsuda, Tomoki ;   Noguchi, Tomoaki ;   Ishihara, Yasutoshi

Tags DiagnosticsNanotechnologyMicrofluidics

Magnetic particle imaging is a medical imaging technology suitable for noninvasive diagnostics. However, the image resolution was degraded due...

Read More Download this Paper

An iPS-derived, Vascularized Cardiac Tissue as an in vitro Drug Screening Platform

Kurokawa, Yosuke ;   Tran, David ;   Moya, Monica ;   Sobrino, Agua, Agua ;   Alonzo, Luis ;   Tu, Christina ;   Lock, Leslie ;   Hughes, Christopher ;   Conklin, Bruce ;   George, Steven

Tags Tissue engineeringStem cellsMicrofluidics

Drug attrition due to cardiac toxicity is prevalent, and a better preclinical screening tool is required in order to solve this issue. Here we...

Read More Download this Paper

Bioluminescence Verification for Droplets Mixing Device Using Electro-Conjugate Fluid

Kobayashi, Kiho ;   Takemura, Kenjiro ;   Yokota, Shinichi ;   Edamura, Kazuya

Tags Lab-on-ChipMicrofluidicsPoint-of-care

Our previous study proposed a mixing device for droplet µTAS using an electro-conjugate fluid. An ECF is a type of functional fluid that...

Read More Download this Paper

Compartmentalized Single Cell Analysis – Antibiotic Susceptibility and Protein Screening

Andersson Svahn, Helene

Tags MicrofluidicsLab-on-ChipDiagnostics

Droplet microfluidics is a technological platform that utilizes monodisperse aqueous droplets in a continuous biocompatible oil phase as...

Read More Download this Paper

Continuous Monitoring of Bacteria in Drinking Water

Svendsen, Winnie E ;   Bertelsen, Christian Vinther ;   Skands, Gustav ;   Dimaki, Maria ;   Clausen, Casper Hyttel

Tags NanotechnologyGlobal healthMicrofluidics

Real-time continuous monitoring of bacteria concentrations in drinking water is of great importance in drinking water management. The methods...

Read More Download this Paper

Design and Simulation of Device for Breach Microfluidics Membrane for the Detection of Hiv (aids)

Lagos, Jose ;   Leguizamon, Ana ;   Quiroga, Daniel

Tags DiagnosticsLab-on-ChipMicrofluidics

This article present a model of diagnostic device that using chemical reagents and microfluidics as particle separation is developed to...

Read More Download this Paper

Development of Brain-on-a-Chip System based on Microfluidic Perfusion of Organotypic Hippocampal Cultures

Liu, Jing ;   Berdichevsky, Yevgeny

Tags Lab-on-ChipMicrofluidicsMicrofabrication

We developed a microfluidic perfusion system to maintain viable organotypic hippocampal cultures. This system is compatible with multiple...

Read More Download this Paper

Digital and Multiplexed Protein Quantification by Microfluidic Very Large Scale Integration

Araci, Ismail Emre ;   Robles, Michael C. ;   Quake, Stephen

Tags Lab-on-ChipMicrofluidicsMicrofabrication

We have designed and fabricated a reusable digital protein detection platform and demonstrated its usage for b-galactosidase expression...

Read More Download this Paper

Droplet Libraries for Digital PCR with Improved Dynamic Range

Kaminski, Tomasz S. ;   Postek, Witold ;   Debski, Pawel R. ;   Garstecki, Piotr

Tags MicrofluidicsLab-on-ChipDiagnostics

This paper reports a microfluidic system that i) generate a sequence of 20 microliter droplets of a 2-fold dilution of DNA concentration ii)...

Read More Download this Paper

Electrical Pacing of 3D Ips-Derived Cardiomyocytes and Endothelial Cells in a Microfluidic Device

Lam, Sandra ;   Simon, Melinda ;   Tran, David ;   Alonzo, Luis ;   Flohn, Nicholas ;   Lee, Abraham ;   George, Steven

Tags MicrofluidicsStem cellsTissue engineering

Tissue engineering models of human tissue have frequently utilized microfabricated systems to accurately and consistently provide simultaneous...

Read More Download this Paper

Electrical Phenotypes and Polymyxin Resistance of Pseudomonas Aeruginosa Clinical Isolates

Wang, Qianru ;   Moskowitz, Samuel ;   Buie, Cullen Richard

Tags MicrofluidicsDiagnostics

Recently we've demonstrated insulator based dielectrophoresis with strain-level discrimination of the opportunistic human pathogen Pseudomonas...

Read More Download this Paper

Fabrication of Biomimetic 3D Liver Tissue by Photodegradable Hydrogels and Perfusion Culture in a Microfluidic Device

Yanagawa, Fumiki ;   Sugiura, Shinji ;   Takagi, Toshiyuki ;   Sumaru, Kimio ;   Kanamori, Toshiyuki

Tags MicrofluidicsTissue engineeringMicrofabrication

This paper reports a novel technique to fabricate perfusable 3D liver tissue in a microfluidic device using photodegradable hydrogels prepared...

Read More Download this Paper

Flexible Microfluidics for CD4+ T-Cell Capture and Counting

Asghar, Waseem ;   Yuksekkaya, Mehmet ;   H. Zhang, Michael ;   shafiee, Hadi ;   Demirci, Utkan

Tags Global healthMicrofluidicsPoint-of-care

There is a significant interest in developing disposable and inexpensive platforms for disease diagnostic and monitoring at point of care (POC)...

Read More Download this Paper

Formation of Lipid Bilayers and Screening of Activity of Membrane Proteins in an Automated Droplet Microfluidic System

Czekalska, Magdalena A. ;   Kaminski, Tomasz S. ;   Jakiela, Slawomir ;   Sapra, K. Tanuj ;   Opalski, Adam S. ;   Bayley, Hagan ;   Garstecki, Piotr

Tags MicrofluidicsLab-on-Chip

This paper reports a microfluidic system that automates the formation of lipid bilayers at the interface of nanoliter aqueous droplets...

Read More Download this Paper

Formation of Spatially-Defined Heterogeneous ECM-Coated Surfaces Using Microfluidic Patterning with Capillary Stop Valves to Control Cell Adhesion of Stem Cells

Kaneda, Shohei ;   Nakamura, Hiroko ;   Kawada, Jiro ;   Ono, Koichi ;   Fujii, Teruo

Tags Stem cellsTissue engineeringMicrofluidics

A microfluidic device to control condition of cell adhesiveness by the surface coated with two different extracellular matrix (ECM) proteins...

Read More Download this Paper

High Throughout, Microprinting of Tumor Spheroids for Anti-Cancer Drug Screening

Stephanie, Lemmo ;   Atefi, Ehsan ;   Tavana, Hossein

Tags Tissue engineeringMicrofluidicsBionanotechnology

We report a new approach to generating tumor spheroids using a polymeric aqueous two-phase system (ATPS). Cancer cells are confined in an...

Read More Download this Paper

HUVEC-MSC Interactions in the Initiation of Capillary Network Formation

Uwamori, Hiroyuki ;   Sudo, Ryo

Tags Tissue engineeringBioMEMSMicrofluidics

There is a demand for construction of functional capillaries in the field of tissue engineering. However, the mechanism of such capillary...

Read More Download this Paper

In Vitro Evaluation of Catheter Design for an Implantable Solid Drug Delivery Pump

Shahhaidar, Ehsaneh ;   Horlen, Kyle ;   Morales, Mercedes ;   Spehr, Paul ;   Morgan, Philip ;   Imran, Mir ;   Fischer, Elmar ;   Schaefbauer, Alyssa ;   Beraki, Simret

Tags Point-of-careMicrofluidicsMicrofabrication

A fully implantable automatic solid drug delivery device offers unique treatment options to patients suffering from chronic conditions. One...

Read More Download this Paper

Interactions between Engineered HBV-Specific T Cells and Hepatocellular Carcinoma Cells in a 3D Microfluidic Platform

Pavesi, Andrea ;   Tanoto, Tan Anthony ;   Chia, Adeline ;   antonio, bertoletti ;   Kamm, Roger D.

Tags MicrofluidicsLab-on-Chip

We present a microfluidic device as efficient pre-clinical model for testing T-cells functionality for personalized medicine.

Read More Download this Paper

Investigating the Role of M2a Polarized Macrophages on Carcinoma Cell Aggregates Dispersion in a 3D Microfluidic Device

Adriani, Giulia ;   Bai, Jing ;   Dang, Truong-Minh ;   Tu, Ting-Yuan ;   Wong, Siew-Cheng ;   Thiery, Jean Paul ;   Kamm, Roger D.

Tags MicrofluidicsLab-on-Chip

In this study, we present a microfluidic platform to investigate macrophage-cancer cell aggregates interactions. In particular, we investigate...

Read More Download this Paper

Methacrylated Gelatin Hydrogel Fibers with Micro-Grooves for Tissues Engineering

Ostrovidov, Serge ;   Shi, Xuetao ;   Khademhosseini, Ali

Tags Tissue engineeringMicrofluidicsRegenerative medicine

Fiber shape processed biomaterials have attracted interest from the tissue engineering community due to their versatile processability and...

Read More Download this Paper

Microfluidic Based Detection of HepA, HepB, and HepC Using Fluorescent Silver Nanocluster Probes (AgNC-DNA)

Del Bonis-O'Donnell, Jackson Travis ;   Fygenson, Deborah ;   Pennathur, Sumita

Tags BionanotechnologyMicrofluidics

Fluorescent silver nanocluster DNA probes (AgNC-DNA) are a new class of fluorophores with emerging applications in biology and medicine. Using...

Read More Download this Paper

Microfluidic Cell-Sized Vesicles for Use as Artificial Antigen Presenting Cells

Vallejo, Derek ;   Lee, Abraham

Tags MicrofluidicsLab-on-Chip

We present a microfluidic platform for continuous generation of stable and monodisperse lipid vesicles without the use of non-biocompatible,...

Read More Download this Paper

Microfluidic Design Advances

Herr, Amy

Tags MicrofluidicsLab-on-ChipBioMEMS

Microfluidic technology has advanced separations science, yet progress in multi-stage electrophoretic separations has lagged. Accepted...

Read More Download this Paper

Microfluidic Devices for Oxygen Control

Eddington, David

Tags MicrofluidicsStem cells

We have developed a suite of tools to control oxygen concentrations in mammalian cell cultures and tissues. These devices leverage the innate...

Read More Download this Paper

Microfluidic Neuron: A Neuromimetic Approach to Interact with Biological Neurons

Levi, Timothee ;   Araki, Ayako ;   Fujii, Teruo

Tags MicrofluidicsBioMEMSLab-on-Chip

This article deals with the design of a new microfluidic device which reproduces the electrical behavior of one biological neuron. The main goal...

Read More Download this Paper

Microfluidic Neuronal Cell-Culture Device for Continuous Measurement of Neurotransmitters Upon Stimulations

Talaei, Sara ;   Simi, Alessandro ;   Bosca, Alessandro ;   Berdondini, Luca ;   van der Wal, Peter D. ;   de Rooij, Nico F.

Tags BioMEMSLab-on-ChipMicrofluidics

We present a compartmentalized neuronal cell-culture device that provides the possibility of obtaining samples directly from the synaptic...

Read More Download this Paper

Microfluidic Single Sperm Entrapment and Analysis

de Wagenaar, Bjorn ;   Berendsen, Jorien ;   Huisintveld, Nik ;   Olthuis, Wouter ;   van den Berg, Albert ;   Segerink, Loes Irene

Tags MicrofluidicsBionanotechnologyDiagnostics

This abstract describes the hydrodynamic trapping of single spermatozoa in a microfluidic platform, allowing the investigation of sperm...

Read More Download this Paper

Microfluidic, Multifunctional, Multilayer, Monodisperse Microbubbles

Lee, Abraham ;   Hettiarachchi, Kanaka ;   Bardin, David ;   Shih, Roger

Tags MicrofluidicsNanomedicineDiagnostics

In this talk I will review my lab's development of microfluidic technology for multilayer vesicles (MVs), focusing on gaseous-cored microbubbles....

Read More Download this Paper

Micropatterning of Cells into Microwells for Metabolic Profiling

Wang, Kuo-chen ;   Meldrum, Deirdre ;   Wang, Hong ;   Chao, Shih-Hui ;   Tian, Yanqing ;   Song, Ganquan

Tags MicrofabricationMicrofluidicsLab-on-Chip

We present a photolithography-based method for micropatterning cells into microwells for metabolic profiling.

Read More Download this Paper

Microphysiological Systems to Study Metastasis at High Spatiotemporal Resolution

Shirure, Venktesh ;   Waterman, Marian ;   George, Steven

Tags Tissue engineeringLab-on-ChipMicrofluidics

Metastasis, the most devastating consequence of cancer, remains challenging to treat, and better understanding of the metastatic process is...

Read More Download this Paper

MIDAS – MIcrofluidics Detection Using Acoustic Spectroscopy

Sherman, Samuel ;   Anstett, Florent ;   Napoli, Maria ;   Bamieh, Bassam ;   Turner, Kimberly ;   Pennathur, Sumita

Tags BioMEMSMicrofluidicsLab-on-Chip

We propose a novel, microfluidics-based device that senses biomolecular markers and viscoelastic properties of relevant bodily fluids by...

Read More Download this Paper

Multimodal Size-Based Microfluidic Sorters

Papautsky, Ian ;   Wang, Xiao

Tags MicrofluidicsLab-on-Chip

Microfluidic sorting of cells has attracted much interest in recent years. However, most microfluidic devices are only capable of bimodal...

Read More Download this Paper

Multiplexed SERS for Rare Cell Detection in Flow

Hoonejani, Mehran ;   Pallaoro, Alessia ;   Braun, Gary ;   Moskovits, Martin ;   Meinhart, Carl

Tags NanotechnologyMicrofluidicsDiagnostics

The reliable and fast identification of different types of cells is of utmost importance in several biomedical applications. The detection of...

Read More Download this Paper

Optical Ph Monitoring for Microfluidic Tissue Culture Applications

Mousavi Shaegh, Seyed Ali ;   Zhang, Yu Shrike ;   Nadhman, Akhtar ;   Khademhosseini, Ali ;   Dokmeci, Mehmet R.

Tags MicrofluidicsTissue engineeringLab-on-Chip

We have developed an optical sensor for monitoring of pH value for microfluidic tissue culture applications using the color change of phenol...

Read More Download this Paper

Physical Phenotyping of Single-Cells for Biomedicine

Di Carlo, Dino

Tags MicrofluidicsStem cellsDiagnostics

Physical properties of cells can provide integrative and quantitative information about various disease states. I will discuss a range of...

Read More Download this Paper

Portable Smartphone-Enabled DNA Analysis for POC Diagnostics

Ugaz, Victor

Tags Point-of-careGlobal healthMicrofluidics

The lack of affordable, rapid, and easy to use POC diagnostic technologies is one of the most critical issues confronting global public health....

Read More Download this Paper

Probing Biophysical Characteristics of Red Blood Cells in Microvasculature Mimicking Microfluidic Channels

Alapan, Yunus ;   Little, Jane ;   Gurkan, Umut A.

Tags MicrofluidicsLab-on-ChipPoint-of-care

Red blood cell (RBC) adhesion and deformability are critical biophysical factors in vaso-occlusion and correlate with sickle cell disease (SCD)...

Read More Download this Paper

Programmable Microfluidic Digital Logic for the Autonomous Lab-on-a-Chip

Ahrar, Siavash ;   Duncan, Philip ;   Raje, Manasi ;   Hui, Elliot

Tags MicrofluidicsLab-on-ChipPoint-of-care

We present sequential logic circuits implemented in microfluidics rather than electronics. A programmable finite state machine was constructed...

Read More Download this Paper

Real-Time Detection and Closed Loop Feedback of Biomolecules in Live Animals

Soh, H. Tom

Tags DiagnosticsPoint-of-careMicrofluidics

A biosensor capable of continuously measuring specific molecules in the bloodstream in vivo would give clinicians a valuable window into...

Read More Download this Paper

Single-Position Inertial Focuser for Sheathless Flow Cytometry

Wang, Xiao ;   Kaval, Necati ;   Papautsky, Ian

Tags MicrofluidicsLab-on-Chip

We present a simple inertial microfluidic device for single position focusing of cells. This straight inertial microfluidic focuser can be easily...

Read More Download this Paper

Styrofoam for Rapid Fabrication of Biosensors with Tunable Sensitivity and Limit of Detection

Talaei, Sara ;   van der Wal, Peter D. ;   de Rooij, Nico F.

Tags BioMEMSLab-on-ChipMicrofluidics

We present a rapid approach for fabrication of amperometric enzyme-based biosensors. The enzyme immobilization is done in one step by...

Read More Download this Paper

Super-Resolved Micro-Vascular Imaging Using Microbubbles and Machine Learning

Christensen-Jeffries, Kirsten ;   Schirmer, Markus D ;   Browning, Richard ;   Tang, Meng-Xing ;   Dunsby, Christopher ;   Aljabar, Paul ;   Eckersley, Robert John

Tags MicrofluidicsDiagnostics

Recent developments in sub-diffraction ultrasound (US) imaging using clinical US systems has shown the potential to resolve structures on the...

Read More Download this Paper

The Development of an Optopiezoelectric Micropump for Microfluidic Applications

Wang, Hsin-Hu ;   Lee, Chih-Kung ;   Hsu, Yu-Hsiang

Tags MicrofluidicsMicrofabricationLab-on-Chip

In this paper, we present a polymer-based piezoelectric micropump that can be selectively activated and controlled by a masked light source....

Read More Download this Paper

Towards a Diffusion-Based Microfluidic Device for Isolation of Cell-Derived Microparticles

Cherré, Solène ;   Olsen, Mark ;   Noemi, Rozlosnik

Tags MicrofluidicsDiagnosticsNanomedicine

Cell-derived microparticles (MPs) have been proposed as novel diagnostics biomarkers for a wide range of diseases. However instruments and...

Read More Download this Paper