3D Microelectrodes on Chip for Impedance-Based Cell Detection and Differentiation

Rollo, Enrica ;   Wilhelm, Jannick ;   Guiducci, Carlotta

Tags MicrofabricationLab-on-ChipMicrofluidics

In this work we show single-particle differentiation in flow based on size and cell state by means of on-chip 50 µm high 3D silicon micropillar...

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A Fabrication Platform for Stretchable Electronic Medical Devices

Savov, Angel ;   Khoshfetrat Pakazad, Saeed ;   Joshi, Shivani ;   Henneken, Vincent ;   Dekker, Ronald

Tags MicrofabricationBioMEMSLab-on-Chip

A CMOS compatible post-processing technology for the fabrication of high-density stretchable electronic circuits is demonstrated. The...

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A Medium Throughput Device to Study the Effects of Combinations of Surface Strains and Fluid-Flow Shear Stresses on Cells

Sinha, Ravi ;   Le Gac, Séverine ;   Verdonschot, Nico ;   van den Berg, Albert ;   Koopman, Bart ;   Rouwkema, Jeroen

Tags Regenerative medicineLab-on-ChipMicrofluidics

We report a 96-well-plate-sized device to screen for the effects on cells of all combinations of five equibiaxial surface strains (2-20%...

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A Microfluidic Device to Investigate the Effect of Alternating Electric Field on Cancer Cells

Pavesi, Andrea ;   Adriani, Giulia ;   Ping, Andy T.K ;   Warkiani, Majid E. ;   Kamm, Roger D.

Tags MicrofluidicsLab-on-Chip

We present a microfluidic device to investigate the effect of electric field on cancer cells proliferation and migration in a 3D matrix and in...

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A Microfluidic Platform for Quantitative Analysis of Cancer Angiogenesis and Intravasation

Lee, Hyunjae ;   Park, Woohyun ;   Ryu, Hyunryul ;   Jeon, Noo Li

Tags BioMEMSLab-on-Chip

Understanding the mechanism behind cancer metastasis is a major challenge in cancer biology. Several in vitro models have been developed to...

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A Microfluidic Platform to Study and Modulate the Blood-Brain Barrier

van der Helm, Marieke Willemijn ;   Frimat, Jean-Philippe ;   Odijk, Mathieu ;   van den Berg, Albert ;   Segerink, Loes Irene

Tags Lab-on-ChipBioMEMSMicrofluidics

A simple yet predictive model of the blood-brain barrier (BBB) is required to help drug development and improve the understanding of the...

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All-Polymer, High-Aspect-Ratio, Soft Lithography

Bubendorfer, Andrea ;   Best, Andrew ;   Arnold, William Michael

Tags MicrofabricationLab-on-ChipBioMEMS

Microfluidic ?chips? are commonly produced by molding or embossing from master structures: the masters need to be able to withstand many...

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An Integrated Bubble Trap for Continuous Removal of Gas Bubbles from Microfluidic Chips

Dokmeci, Mehmet R. ;   Zhang, Yu Shrike ;   Chae, Sukyoung ;   Polini, Alessandro ;   Khademhosseini, Ali

Tags BioMEMSLab-on-Chip

In this paper we present a miniature bubble trap for efficient removal of bubbles from microfluidic reactors. The new bubble trap has a 10 mm...

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Application of Silicon Microwire Arrays in Characterization of Skeletal Muscle Tissue

Banan Sadeghian, Ramin ;   Ostrovidov, Serge ;   Oi, Hideo ;   Kawano, Takeshi ;   Khademhosseini, Ali

Tags Tissue engineeringMicrofabricationLab-on-Chip

We report on the establishment of skeletal muscle cell cultures on silicon (Si) microwire arrays grown by a vapor-liquid-solid (VLS) technique....

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Assessments of T-Nanowire Transistor Assay for Clinical Diabetic Nephropathy

Mao, Yufei ;   Shin, Kyeong-Sik ;   Nicholas, Susanne ;   Chui, Chi On

Tags DiagnosticsLab-on-ChipBionanotechnology

For the first time, we assessed the amplifying T-nanowire transistor assay in quantitating a key diabetic kidney disease (DKD) urinary...

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Bioluminescence Verification for Droplets Mixing Device Using Electro-Conjugate Fluid

Kobayashi, Kiho ;   Takemura, Kenjiro ;   Yokota, Shinichi ;   Edamura, Kazuya

Tags Lab-on-ChipMicrofluidicsPoint-of-care

Our previous study proposed a mixing device for droplet µTAS using an electro-conjugate fluid. An ECF is a type of functional fluid that...

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Charge Coupled pH Monitoring in Thin-Film Dual-Gated ISFETs

Duarte-Guevara, Carlos ;   Lai, Fei-Lung ;   Reddy, Bobby ;   Salm, Eric ;   Vilasur Swaminathan, Vikhram ;   Liu, Yi-Shao ;   Bashir, Rashid

Tags DiagnosticsLab-on-ChipPoint-of-care

Reference electrodes, that are required for the operation of ion-sensitive field-effect transistors, are unreliable and too large for droplet...

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Compartmentalized Single Cell Analysis – Antibiotic Susceptibility and Protein Screening

Andersson Svahn, Helene

Tags MicrofluidicsLab-on-ChipDiagnostics

Droplet microfluidics is a technological platform that utilizes monodisperse aqueous droplets in a continuous biocompatible oil phase as...

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Design and Simulation of Device for Breach Microfluidics Membrane for the Detection of Hiv (aids)

Lagos, Jose ;   Leguizamon, Ana ;   Quiroga, Daniel

Tags DiagnosticsLab-on-ChipMicrofluidics

This article present a model of diagnostic device that using chemical reagents and microfluidics as particle separation is developed to...

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Detection of PCR Amplification by Top-Down Silicon Nanowires Field Effect Transistors

Accastelli, Enrico ;   Spiga, Fabio Mario ;   Ernst, Thomas ;   Guiducci, Carlotta

Tags Point-of-careMicrofabricationLab-on-Chip

We propose the implementation of PCR system measuring DNA amplification by using top-down, CMOS compatible SiNWs. The DNA amplification cycles...

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Development of Brain-on-a-Chip System based on Microfluidic Perfusion of Organotypic Hippocampal Cultures

Liu, Jing ;   Berdichevsky, Yevgeny

Tags Lab-on-ChipMicrofluidicsMicrofabrication

We developed a microfluidic perfusion system to maintain viable organotypic hippocampal cultures. This system is compatible with multiple...

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Diabetachip: Organ-on-Chip for a Novel Cell-Based Biosensor in Pretransplantation Quality Control and in Treatment of Type 1 Diabetes

Raoux, Matthieu ;   Lebreton, Fanny ;   Pedraza, Eileen ;   Bornat, Yannick ;   Floderer, Jean-Baptiste ;   Catargi, Bogdan ;   Renaud, Sylvie ;   Lang, Jochen

Tags BioMEMSLab-on-ChipGlobal health

Continuous glucose monitoring sensors only recognize glucose but no other hormones, they can therefore not work as a closed loop and...

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Digital and Multiplexed Protein Quantification by Microfluidic Very Large Scale Integration

Araci, Ismail Emre ;   Robles, Michael C. ;   Quake, Stephen

Tags Lab-on-ChipMicrofluidicsMicrofabrication

We have designed and fabricated a reusable digital protein detection platform and demonstrated its usage for b-galactosidase expression...

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Droplet Libraries for Digital PCR with Improved Dynamic Range

Kaminski, Tomasz S. ;   Postek, Witold ;   Debski, Pawel R. ;   Garstecki, Piotr

Tags MicrofluidicsLab-on-ChipDiagnostics

This paper reports a microfluidic system that i) generate a sequence of 20 microliter droplets of a 2-fold dilution of DNA concentration ii)...

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Effect of the Flexural Rigidity of a Piezoelectric Thin-Film on the Neonatal Rat Cardiomyocytes Maturation

Hsu, Yu-Hsiang ;   Lin, Yu-Huan ;   Chou, Han-Yi E. ;   Chen, Li-Yu ;   Shen, Jia-Wei

Tags BioMEMSLab-on-ChipTissue engineering

In this paper, a piezoelectric Polyvinylidene fluoride (PVDF) transducer for the mechanical detection of cardiomycytes is presented. In order...

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Flexible Strain Sensors for Measuring Contractility Force of Human Stem Cell-Derived Cardiomyocytes

Mendoza, Nicole ;   Carlos, Ruiz ;   Tang, William C. ;   Khine, Michelle

Tags Stem cellsLab-on-ChipMicrofabrication

Contractility assessment of stem cell-derived cardiomyocytes is required to characterize their maturity and functional behavior in vitro. We...

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Formation of Lipid Bilayers and Screening of Activity of Membrane Proteins in an Automated Droplet Microfluidic System

Czekalska, Magdalena A. ;   Kaminski, Tomasz S. ;   Jakiela, Slawomir ;   Sapra, K. Tanuj ;   Opalski, Adam S. ;   Bayley, Hagan ;   Garstecki, Piotr

Tags MicrofluidicsLab-on-Chip

This paper reports a microfluidic system that automates the formation of lipid bilayers at the interface of nanoliter aqueous droplets...

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HemeChip for Early Diagnosis of Sickle Cell Disease in Newborns

Alapan, Yunus ;   Ung, Ryan ;   Romelfanger, Megan ;   Piccone, Connie ;   Little, Jane ;   Gurkan, Umut A.

Tags DiagnosticsPoint-of-careLab-on-Chip

More than 800 children are born with Sickle Cell Disease (SCD) every day in Africa, and more than half of them die in childhood due to lack of...

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Interactions between Engineered HBV-Specific T Cells and Hepatocellular Carcinoma Cells in a 3D Microfluidic Platform

Pavesi, Andrea ;   Tanoto, Tan Anthony ;   Chia, Adeline ;   antonio, bertoletti ;   Kamm, Roger D.

Tags MicrofluidicsLab-on-Chip

We present a microfluidic device as efficient pre-clinical model for testing T-cells functionality for personalized medicine.

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Investigating the Role of M2a Polarized Macrophages on Carcinoma Cell Aggregates Dispersion in a 3D Microfluidic Device

Adriani, Giulia ;   Bai, Jing ;   Dang, Truong-Minh ;   Tu, Ting-Yuan ;   Wong, Siew-Cheng ;   Thiery, Jean Paul ;   Kamm, Roger D.

Tags MicrofluidicsLab-on-Chip

In this study, we present a microfluidic platform to investigate macrophage-cancer cell aggregates interactions. In particular, we investigate...

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Mechanical Force Induced Transition of Cell Protrusions from Blebs to Lamellipodia

Yeh, Chuan-Feng ;   Juang, Duane ;   Hsu, Chia-Hsien

Tags MicrofabricationLab-on-ChipBioMEMS

Cancer cells exhibit highly diverse types of membrane protrusions during metastasis and invasion1. Both chemical2 and physical signals in the...

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Microfluidic Cell-Sized Vesicles for Use as Artificial Antigen Presenting Cells

Vallejo, Derek ;   Lee, Abraham

Tags MicrofluidicsLab-on-Chip

We present a microfluidic platform for continuous generation of stable and monodisperse lipid vesicles without the use of non-biocompatible,...

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Microfluidic Design Advances

Herr, Amy

Tags MicrofluidicsLab-on-ChipBioMEMS

Microfluidic technology has advanced separations science, yet progress in multi-stage electrophoretic separations has lagged. Accepted...

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Microfluidic Neuron: A Neuromimetic Approach to Interact with Biological Neurons

Levi, Timothee ;   Araki, Ayako ;   Fujii, Teruo

Tags MicrofluidicsBioMEMSLab-on-Chip

This article deals with the design of a new microfluidic device which reproduces the electrical behavior of one biological neuron. The main goal...

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Microfluidic Neuronal Cell-Culture Device for Continuous Measurement of Neurotransmitters Upon Stimulations

Talaei, Sara ;   Simi, Alessandro ;   Bosca, Alessandro ;   Berdondini, Luca ;   van der Wal, Peter D. ;   de Rooij, Nico F.

Tags BioMEMSLab-on-ChipMicrofluidics

We present a compartmentalized neuronal cell-culture device that provides the possibility of obtaining samples directly from the synaptic...

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Micropatterning of Cells into Microwells for Metabolic Profiling

Wang, Kuo-chen ;   Meldrum, Deirdre ;   Wang, Hong ;   Chao, Shih-Hui ;   Tian, Yanqing ;   Song, Ganquan

Tags MicrofabricationMicrofluidicsLab-on-Chip

We present a photolithography-based method for micropatterning cells into microwells for metabolic profiling.

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Microphysiological Systems to Study Metastasis at High Spatiotemporal Resolution

Shirure, Venktesh ;   Waterman, Marian ;   George, Steven

Tags Tissue engineeringLab-on-ChipMicrofluidics

Metastasis, the most devastating consequence of cancer, remains challenging to treat, and better understanding of the metastatic process is...

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MIDAS – MIcrofluidics Detection Using Acoustic Spectroscopy

Sherman, Samuel ;   Anstett, Florent ;   Napoli, Maria ;   Bamieh, Bassam ;   Turner, Kimberly ;   Pennathur, Sumita

Tags BioMEMSMicrofluidicsLab-on-Chip

We propose a novel, microfluidics-based device that senses biomolecular markers and viscoelastic properties of relevant bodily fluids by...

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Multimodal Size-Based Microfluidic Sorters

Papautsky, Ian ;   Wang, Xiao

Tags MicrofluidicsLab-on-Chip

Microfluidic sorting of cells has attracted much interest in recent years. However, most microfluidic devices are only capable of bimodal...

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NeuroChip: Bottoms Up Discovery Platform for Neuronal Development and Disorders

Thakor, Nitish

Tags Lab-on-ChipBioMEMSTissue engineering

The NeuroChip platform enables isolation and patterning of neuronal constituents (soma, axons, neurites) as well as co-culture of...

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Optical Ph Monitoring for Microfluidic Tissue Culture Applications

Mousavi Shaegh, Seyed Ali ;   Zhang, Yu Shrike ;   Nadhman, Akhtar ;   Khademhosseini, Ali ;   Dokmeci, Mehmet R.

Tags MicrofluidicsTissue engineeringLab-on-Chip

We have developed an optical sensor for monitoring of pH value for microfluidic tissue culture applications using the color change of phenol...

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Perfusion-Based Three Dimensional (3D) Tissue Engineering Platform with Integrated Bioimpedance Sensing

Muhammad, Haseena Bashir ;   Canali, Chiara ;   Heiskanen, Arto ;   Hemmingsen, Mette ;   Wolff, Anders ;   Dufva, Martin ;   Emnéus, Jenny

Tags Tissue engineeringLab-on-Chip

We present an 8-channel bioreactor array with integrated bioimpedance sensors, which enables perfusion culture of cells seeded onto porous...

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Probing Biophysical Characteristics of Red Blood Cells in Microvasculature Mimicking Microfluidic Channels

Alapan, Yunus ;   Little, Jane ;   Gurkan, Umut A.

Tags MicrofluidicsLab-on-ChipPoint-of-care

Red blood cell (RBC) adhesion and deformability are critical biophysical factors in vaso-occlusion and correlate with sickle cell disease (SCD)...

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Programmable Microfluidic Digital Logic for the Autonomous Lab-on-a-Chip

Ahrar, Siavash ;   Duncan, Philip ;   Raje, Manasi ;   Hui, Elliot

Tags MicrofluidicsLab-on-ChipPoint-of-care

We present sequential logic circuits implemented in microfluidics rather than electronics. A programmable finite state machine was constructed...

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Real Time Impedance Sensing of Intermediate Stages of Mitosis of a Single Cell

Kaji, Hirokazu ;   Ghenim, Lamya ;   Nagamine, Kuniaki ;   Porte, Stephanie ;   Haguet, Vincent ;   Gidrol, Xavier ;   Nishizawa, Matsuhiko

Tags Lab-on-ChipDiagnostics

We have constructed a sensitive tool to monitor electrically changes of individual cells by combining electrochemical-based surface modification...

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Single-Position Inertial Focuser for Sheathless Flow Cytometry

Wang, Xiao ;   Kaval, Necati ;   Papautsky, Ian

Tags MicrofluidicsLab-on-Chip

We present a simple inertial microfluidic device for single position focusing of cells. This straight inertial microfluidic focuser can be easily...

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Stereolithographic Manufacture of Microneedle Patch Arrays for Blood Collection

Faraji Rad, Zahra ;   Davies, Graham ;   Anthony, Carl ;   Prewett, Philip D ;   Nordon, Robert E

Tags MicrofabricationDiagnosticsLab-on-Chip

3D laser lithography is used to manufacture microneedle master molds with optimal geometric designs for collection of subcutaneous capillary...

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Styrofoam for Rapid Fabrication of Biosensors with Tunable Sensitivity and Limit of Detection

Talaei, Sara ;   van der Wal, Peter D. ;   de Rooij, Nico F.

Tags BioMEMSLab-on-ChipMicrofluidics

We present a rapid approach for fabrication of amperometric enzyme-based biosensors. The enzyme immobilization is done in one step by...

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The Development of an Optopiezoelectric Micropump for Microfluidic Applications

Wang, Hsin-Hu ;   Lee, Chih-Kung ;   Hsu, Yu-Hsiang

Tags MicrofluidicsMicrofabricationLab-on-Chip

In this paper, we present a polymer-based piezoelectric micropump that can be selectively activated and controlled by a masked light source....

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