3D-Bioengineering of the Conventional Outflow Tract as Model for High-Throughput Drug Screening

Cula, Dautriche ;   Susan, Sharfstein ;   Xie, Yubing ;   Bergkvist, Magnus ;   Kerr, Matthew ;   Szymanski, Dennis ;   Danias, John ;   Stamer, W. D.

Tags Tissue engineeringRegenerative medicineMicrofabrication

Glaucoma remains the leading cause of irreversible blindness worldwide. Glaucoma is characterized by chronic degeneration of axons of the...

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An Engineered Microfluidic Device for Modeling Cardiac Tissues

Acun, Aylin ;   Zorlutuna, Pinar

Tags MicrofluidicsStem cellsTissue engineering

Studying the heart tissue is critical for understanding and developing treatments for cardiovascular diseases. In this work, we present a...

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An iPS-derived, Vascularized Cardiac Tissue as an in vitro Drug Screening Platform

Kurokawa, Yosuke ;   Tran, David ;   Moya, Monica ;   Sobrino, Agua, Agua ;   Alonzo, Luis ;   Tu, Christina ;   Lock, Leslie ;   Hughes, Christopher ;   Conklin, Bruce ;   George, Steven

Tags Tissue engineeringStem cellsMicrofluidics

Drug attrition due to cardiac toxicity is prevalent, and a better preclinical screening tool is required in order to solve this issue. Here we...

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Application of Silicon Microwire Arrays in Characterization of Skeletal Muscle Tissue

Banan Sadeghian, Ramin ;   Ostrovidov, Serge ;   Oi, Hideo ;   Kawano, Takeshi ;   Khademhosseini, Ali

Tags Tissue engineeringMicrofabricationLab-on-Chip

We report on the establishment of skeletal muscle cell cultures on silicon (Si) microwire arrays grown by a vapor-liquid-solid (VLS) technique....

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Cytodetachment Technique Using Ultrasonic Vibration

Kurashina, Yuta ;   Takemura, Kenjiro ;   Miyata, Shogo ;   Komotori, Jun ;   Koyama, Tadayoshi

Tags BionanotechnologyMicrofabricationTissue engineering

Ultrasonic vibration is often used in tissue engineering, for example, to provide effective mechanical stimuli for cell growth. This study aims...

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Effect of the Flexural Rigidity of a Piezoelectric Thin-Film on the Neonatal Rat Cardiomyocytes Maturation

Hsu, Yu-Hsiang ;   Lin, Yu-Huan ;   Chou, Han-Yi E. ;   Chen, Li-Yu ;   Shen, Jia-Wei

Tags BioMEMSLab-on-ChipTissue engineering

In this paper, a piezoelectric Polyvinylidene fluoride (PVDF) transducer for the mechanical detection of cardiomycytes is presented. In order...

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Effect on Invasive Capacity of Cancer Cell Spheroid Co-Cultured with Vascular Endothelial Cell Network Using In Vitro 3D Patterning Model

Yamamoto, Shuhei ;   Okochi, Mina ;   Honda, Hiroyuki

Tags BionanotechnologyTissue engineering

In vitro three dimensional (3D) cancer models were developed to observe the invasive capacity of cancer cell spheroids co-cultured with the...

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Elasticity Tunable Hybrid Hydrogels Using Light Irradiation

Yanagawa, Fumiki ;   Mizutani, Takeomi ;   Sugiura, Shinji ;   Takagi, Toshiyuki ;   Sumaru, Kimio ;   Kanamori, Toshiyuki

Tags Tissue engineeringMicrofabrication

This paper reports a novel technique to synthesize elasticity tunable hybrid hydrogels with an activated-ester-type photocleavable...

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Electrical Pacing of 3D Ips-Derived Cardiomyocytes and Endothelial Cells in a Microfluidic Device

Lam, Sandra ;   Simon, Melinda ;   Tran, David ;   Alonzo, Luis ;   Flohn, Nicholas ;   Lee, Abraham ;   George, Steven

Tags MicrofluidicsStem cellsTissue engineering

Tissue engineering models of human tissue have frequently utilized microfabricated systems to accurately and consistently provide simultaneous...

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Fabrication of Biomimetic 3D Liver Tissue by Photodegradable Hydrogels and Perfusion Culture in a Microfluidic Device

Yanagawa, Fumiki ;   Sugiura, Shinji ;   Takagi, Toshiyuki ;   Sumaru, Kimio ;   Kanamori, Toshiyuki

Tags MicrofluidicsTissue engineeringMicrofabrication

This paper reports a novel technique to fabricate perfusable 3D liver tissue in a microfluidic device using photodegradable hydrogels prepared...

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Formation of Spatially-Defined Heterogeneous ECM-Coated Surfaces Using Microfluidic Patterning with Capillary Stop Valves to Control Cell Adhesion of Stem Cells

Kaneda, Shohei ;   Nakamura, Hiroko ;   Kawada, Jiro ;   Ono, Koichi ;   Fujii, Teruo

Tags Stem cellsTissue engineeringMicrofluidics

A microfluidic device to control condition of cell adhesiveness by the surface coated with two different extracellular matrix (ECM) proteins...

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High Throughout, Microprinting of Tumor Spheroids for Anti-Cancer Drug Screening

Stephanie, Lemmo ;   Atefi, Ehsan ;   Tavana, Hossein

Tags Tissue engineeringMicrofluidicsBionanotechnology

We report a new approach to generating tumor spheroids using a polymeric aqueous two-phase system (ATPS). Cancer cells are confined in an...

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HUVEC-MSC Interactions in the Initiation of Capillary Network Formation

Uwamori, Hiroyuki ;   Sudo, Ryo

Tags Tissue engineeringBioMEMSMicrofluidics

There is a demand for construction of functional capillaries in the field of tissue engineering. However, the mechanism of such capillary...

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Hydrogels Containing Aligned Carbon Nanotubes for Fabrication of Muscle Myofibers and Cardiac Differentiation of Embryoid Bodies

Ahadian, Samad ;   Khademhosseini, Ali

Tags BionanotechnologyTissue engineeringStem cells

Dielectrophoresis (DEP) method was introduced for the fabrication of methacrylated gelatin (GelMA)-aligned carbon nanotube (CNT) gels with...

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Indirect Fabrication of Three-Dimensional Hydrogel Constructs for Tissue Engineering Applications

Pimentel Carletto, Rodrigo ;   Muhammad, Haseena Bashir ;   Wolff, Anders ;   Dufva, Martin ;   Emnéus, Jenny ;   Okkels, Fridolin

Tags Tissue engineeringMicrofabrication

We present a three-dimensional (3D) tissue-engineering construct, realized using an indirect fabrication technique. By using fused deposition...

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Innervation of 3D Printed Biological Machines

Cvetkovic, Caroline ;   Swetenburg, Raymond ;   Rich, Max H ;   Kong, Hyunjoon ;   Stice, Steven L ;   Bashir, Rashid

Tags Tissue engineeringBionanotechnologyStem cells

We report the development of a skeletal muscle powered biological machine (?bio-bot?) capable of biomimetic and directional locomotion, as well...

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Methacrylated Gelatin Hydrogel Fibers with Micro-Grooves for Tissues Engineering

Ostrovidov, Serge ;   Shi, Xuetao ;   Khademhosseini, Ali

Tags Tissue engineeringMicrofluidicsRegenerative medicine

Fiber shape processed biomaterials have attracted interest from the tissue engineering community due to their versatile processability and...

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Microphysiological Systems of Cardiac and Tumor Tissue Derived from Induced Pluripotent Stem Cells

George, Steven

Tags Tissue engineeringBioMEMSStem cells

Cancer remains a leading cause of mortality in the United States, and world wide. There remains a need to develop new therapeutic options....

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Microphysiological Systems to Study Metastasis at High Spatiotemporal Resolution

Shirure, Venktesh ;   Waterman, Marian ;   George, Steven

Tags Tissue engineeringLab-on-ChipMicrofluidics

Metastasis, the most devastating consequence of cancer, remains challenging to treat, and better understanding of the metastatic process is...

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Microplatform to Identify Drug-Induced Cardiotoxicity in IPS-Derived Cardiomyocytes with Bright-Field Imaging and Machine Learning

Lee, Eugene ;   Kurokawa, Yosuke ;   George, Steven ;   Khine, Michelle

Tags Stem cellsTissue engineeringBionanotechnology

Current drug screening methods are ineffective at detecting drug-induced cardiotoxicity. Here we present a detection method that utilizes...

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Microvascular-Like Networks Induced Hydrogel Block for Tissue Engineering

Lee, Kwang-Ho ;   Lee, Hayoung

Tags Tissue engineering

Micro-vasularization is a key factor of tissue regeneration, which play important regulatory roles in regenerating tissue. Combination of...

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Multi-Scale Topographical Patterned Substrates for Promoting Differentiation of Human Neural Stem Cells

Yang, Kisuk ;   Jung, Hyunjung ;   Lee, Hak-Rae ;   Bang, Joona ;   Im, Sung Gap ;   Cho, Seung-Woo

Tags NanotechnologyStem cellsTissue engineering

Self-renewal and differentiation of human neural stem cells (hNSCs) can be controlled by surface topography of culture substrates. In this...

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NeuroChip: Bottoms Up Discovery Platform for Neuronal Development and Disorders

Thakor, Nitish

Tags Lab-on-ChipBioMEMSTissue engineering

The NeuroChip platform enables isolation and patterning of neuronal constituents (soma, axons, neurites) as well as co-culture of...

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Optical Cell Separation from 3D Culture Environment in Photodegradable Hydrogels for Pure Culture Techniques

Tamura, Masato ;   Yanagawa, Fumiki ;   Sugiura, Shinji ;   Takagi, Toshiyuki ;   Sumaru, Kimio ;   Matsui, Hirofumi ;   Kanamori, Toshiyuki

Tags Tissue engineeringDiagnostics

We present a new methodology for optical cell picking in 3D culture environment. In this study, we encapsulated cells in photodegradable...

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Optical Ph Monitoring for Microfluidic Tissue Culture Applications

Mousavi Shaegh, Seyed Ali ;   Zhang, Yu Shrike ;   Nadhman, Akhtar ;   Khademhosseini, Ali ;   Dokmeci, Mehmet R.

Tags MicrofluidicsTissue engineeringLab-on-Chip

We have developed an optical sensor for monitoring of pH value for microfluidic tissue culture applications using the color change of phenol...

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Perfusion-Based Three Dimensional (3D) Tissue Engineering Platform with Integrated Bioimpedance Sensing

Muhammad, Haseena Bashir ;   Canali, Chiara ;   Heiskanen, Arto ;   Hemmingsen, Mette ;   Wolff, Anders ;   Dufva, Martin ;   Emnéus, Jenny

Tags Tissue engineeringLab-on-Chip

We present an 8-channel bioreactor array with integrated bioimpedance sensors, which enables perfusion culture of cells seeded onto porous...

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Projection Micro-Stereolithography Apparatus for High-Resolution 3D Patterning of Cells: Applications in Vascular Tissue Engineering

Raman, Ritu ;   Bhaduri, Basanta ;   Shkumatov, Artem ;   Kong, Hyunjoon ;   Popescu, Gabriel ;   Bashir, Rashid

Tags MicrofabricationBionanotechnologyTissue engineering

We have developed a projection micro-stereolithography apparatus (microSLA) capable of patterning cells encapsulated in biocompatible...

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Three Dimensional Microfluidic Model to Investigate Synergism of Biomechanical and Biochemical Factors on Angiogenic Sprouting

Chung, Minhwan ;   Ahn, Jungho ;   Kim, Sudong ;   Lee, Hyunjae ;   Jeon, Noo Li

Tags BioMEMSTissue engineering

Interstitial fluid is composing more than 20 % of our body fluid, making creeping but constant flow called interstitial flow (IF) through the...

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