3D Microelectrodes on Chip for Impedance-Based Cell Detection and Differentiation

Rollo, Enrica ;   Wilhelm, Jannick ;   Guiducci, Carlotta

Tags MicrofabricationLab-on-ChipMicrofluidics

In this work we show single-particle differentiation in flow based on size and cell state by means of on-chip 50 µm high 3D silicon micropillar...

Read More Download this Paper

A Medium Throughput Device to Study the Effects of Combinations of Surface Strains and Fluid-Flow Shear Stresses on Cells

Sinha, Ravi ;   Le Gac, Séverine ;   Verdonschot, Nico ;   van den Berg, Albert ;   Koopman, Bart ;   Rouwkema, Jeroen

Tags Regenerative medicineLab-on-ChipMicrofluidics

We report a 96-well-plate-sized device to screen for the effects on cells of all combinations of five equibiaxial surface strains (2-20%...

Read More Download this Paper

A Microfluidic Device to Investigate the Effect of Alternating Electric Field on Cancer Cells

Pavesi, Andrea ;   Adriani, Giulia ;   Ping, Andy T.K ;   Warkiani, Majid E. ;   Kamm, Roger D.

Tags MicrofluidicsLab-on-Chip

We present a microfluidic device to investigate the effect of electric field on cancer cells proliferation and migration in a 3D matrix and in...

Read More Download this Paper

An Engineered Microfluidic Device for Modeling Cardiac Tissues

Acun, Aylin ;   Zorlutuna, Pinar

Tags MicrofluidicsStem cellsTissue engineering

Studying the heart tissue is critical for understanding and developing treatments for cardiovascular diseases. In this work, we present a...

Read More Download this Paper

Application of Silicon Microwire Arrays in Characterization of Skeletal Muscle Tissue

Banan Sadeghian, Ramin ;   Ostrovidov, Serge ;   Oi, Hideo ;   Kawano, Takeshi ;   Khademhosseini, Ali

Tags Tissue engineeringMicrofabricationLab-on-Chip

We report on the establishment of skeletal muscle cell cultures on silicon (Si) microwire arrays grown by a vapor-liquid-solid (VLS) technique....

Read More Download this Paper

Development of Brain-on-a-Chip System based on Microfluidic Perfusion of Organotypic Hippocampal Cultures

Liu, Jing ;   Berdichevsky, Yevgeny

Tags Lab-on-ChipMicrofluidicsMicrofabrication

We developed a microfluidic perfusion system to maintain viable organotypic hippocampal cultures. This system is compatible with multiple...

Read More Download this Paper

Diabetachip: Organ-on-Chip for a Novel Cell-Based Biosensor in Pretransplantation Quality Control and in Treatment of Type 1 Diabetes

Raoux, Matthieu ;   Lebreton, Fanny ;   Pedraza, Eileen ;   Bornat, Yannick ;   Floderer, Jean-Baptiste ;   Catargi, Bogdan ;   Renaud, Sylvie ;   Lang, Jochen

Tags BioMEMSLab-on-ChipGlobal health

Continuous glucose monitoring sensors only recognize glucose but no other hormones, they can therefore not work as a closed loop and...

Read More Download this Paper

Effect of the Flexural Rigidity of a Piezoelectric Thin-Film on the Neonatal Rat Cardiomyocytes Maturation

Hsu, Yu-Hsiang ;   Lin, Yu-Huan ;   Chou, Han-Yi E. ;   Chen, Li-Yu ;   Shen, Jia-Wei

Tags BioMEMSLab-on-ChipTissue engineering

In this paper, a piezoelectric Polyvinylidene fluoride (PVDF) transducer for the mechanical detection of cardiomycytes is presented. In order...

Read More Download this Paper

Electrical Pacing of 3D Ips-Derived Cardiomyocytes and Endothelial Cells in a Microfluidic Device

Lam, Sandra ;   Simon, Melinda ;   Tran, David ;   Alonzo, Luis ;   Flohn, Nicholas ;   Lee, Abraham ;   George, Steven

Tags MicrofluidicsStem cellsTissue engineering

Tissue engineering models of human tissue have frequently utilized microfabricated systems to accurately and consistently provide simultaneous...

Read More Download this Paper

Fabrication of Biomimetic 3D Liver Tissue by Photodegradable Hydrogels and Perfusion Culture in a Microfluidic Device

Yanagawa, Fumiki ;   Sugiura, Shinji ;   Takagi, Toshiyuki ;   Sumaru, Kimio ;   Kanamori, Toshiyuki

Tags MicrofluidicsTissue engineeringMicrofabrication

This paper reports a novel technique to fabricate perfusable 3D liver tissue in a microfluidic device using photodegradable hydrogels prepared...

Read More Download this Paper

Flexible Strain Sensors for Measuring Contractility Force of Human Stem Cell-Derived Cardiomyocytes

Mendoza, Nicole ;   Carlos, Ruiz ;   Tang, William C. ;   Khine, Michelle

Tags Stem cellsLab-on-ChipMicrofabrication

Contractility assessment of stem cell-derived cardiomyocytes is required to characterize their maturity and functional behavior in vitro. We...

Read More Download this Paper

Hydrogels Containing Aligned Carbon Nanotubes for Fabrication of Muscle Myofibers and Cardiac Differentiation of Embryoid Bodies

Ahadian, Samad ;   Khademhosseini, Ali

Tags BionanotechnologyTissue engineeringStem cells

Dielectrophoresis (DEP) method was introduced for the fabrication of methacrylated gelatin (GelMA)-aligned carbon nanotube (CNT) gels with...

Read More Download this Paper

Interactions between Engineered HBV-Specific T Cells and Hepatocellular Carcinoma Cells in a 3D Microfluidic Platform

Pavesi, Andrea ;   Tanoto, Tan Anthony ;   Chia, Adeline ;   antonio, bertoletti ;   Kamm, Roger D.

Tags MicrofluidicsLab-on-Chip

We present a microfluidic device as efficient pre-clinical model for testing T-cells functionality for personalized medicine.

Read More Download this Paper

Investigating the Role of M2a Polarized Macrophages on Carcinoma Cell Aggregates Dispersion in a 3D Microfluidic Device

Adriani, Giulia ;   Bai, Jing ;   Dang, Truong-Minh ;   Tu, Ting-Yuan ;   Wong, Siew-Cheng ;   Thiery, Jean Paul ;   Kamm, Roger D.

Tags MicrofluidicsLab-on-Chip

In this study, we present a microfluidic platform to investigate macrophage-cancer cell aggregates interactions. In particular, we investigate...

Read More Download this Paper

Mechanical Force Induced Transition of Cell Protrusions from Blebs to Lamellipodia

Yeh, Chuan-Feng ;   Juang, Duane ;   Hsu, Chia-Hsien

Tags MicrofabricationLab-on-ChipBioMEMS

Cancer cells exhibit highly diverse types of membrane protrusions during metastasis and invasion1. Both chemical2 and physical signals in the...

Read More Download this Paper

Microfluidic Cell-Sized Vesicles for Use as Artificial Antigen Presenting Cells

Vallejo, Derek ;   Lee, Abraham

Tags MicrofluidicsLab-on-Chip

We present a microfluidic platform for continuous generation of stable and monodisperse lipid vesicles without the use of non-biocompatible,...

Read More Download this Paper

Microfluidic Devices for Oxygen Control

Eddington, David

Tags MicrofluidicsStem cells

We have developed a suite of tools to control oxygen concentrations in mammalian cell cultures and tissues. These devices leverage the innate...

Read More Download this Paper

Micropatterning of Cells into Microwells for Metabolic Profiling

Wang, Kuo-chen ;   Meldrum, Deirdre ;   Wang, Hong ;   Chao, Shih-Hui ;   Tian, Yanqing ;   Song, Ganquan

Tags MicrofabricationMicrofluidicsLab-on-Chip

We present a photolithography-based method for micropatterning cells into microwells for metabolic profiling.

Read More Download this Paper

Microphysiological Systems of Cardiac and Tumor Tissue Derived from Induced Pluripotent Stem Cells

George, Steven

Tags Tissue engineeringBioMEMSStem cells

Cancer remains a leading cause of mortality in the United States, and world wide. There remains a need to develop new therapeutic options....

Read More Download this Paper

NeuroChip: Bottoms Up Discovery Platform for Neuronal Development and Disorders

Thakor, Nitish

Tags Lab-on-ChipBioMEMSTissue engineering

The NeuroChip platform enables isolation and patterning of neuronal constituents (soma, axons, neurites) as well as co-culture of...

Read More Download this Paper

Optical Ph Monitoring for Microfluidic Tissue Culture Applications

Mousavi Shaegh, Seyed Ali ;   Zhang, Yu Shrike ;   Nadhman, Akhtar ;   Khademhosseini, Ali ;   Dokmeci, Mehmet R.

Tags MicrofluidicsTissue engineeringLab-on-Chip

We have developed an optical sensor for monitoring of pH value for microfluidic tissue culture applications using the color change of phenol...

Read More Download this Paper

Perfusion-Based Three Dimensional (3D) Tissue Engineering Platform with Integrated Bioimpedance Sensing

Muhammad, Haseena Bashir ;   Canali, Chiara ;   Heiskanen, Arto ;   Hemmingsen, Mette ;   Wolff, Anders ;   Dufva, Martin ;   Emnéus, Jenny

Tags Tissue engineeringLab-on-Chip

We present an 8-channel bioreactor array with integrated bioimpedance sensors, which enables perfusion culture of cells seeded onto porous...

Read More Download this Paper

Physical Phenotyping of Single-Cells for Biomedicine

Di Carlo, Dino

Tags MicrofluidicsStem cellsDiagnostics

Physical properties of cells can provide integrative and quantitative information about various disease states. I will discuss a range of...

Read More Download this Paper

Real Time Impedance Sensing of Intermediate Stages of Mitosis of a Single Cell

Kaji, Hirokazu ;   Ghenim, Lamya ;   Nagamine, Kuniaki ;   Porte, Stephanie ;   Haguet, Vincent ;   Gidrol, Xavier ;   Nishizawa, Matsuhiko

Tags Lab-on-ChipDiagnostics

We have constructed a sensitive tool to monitor electrically changes of individual cells by combining electrochemical-based surface modification...

Read More Download this Paper