Electrical Phenotypes and Polymyxin Resistance of Pseudomonas Aeruginosa Clinical Isolates

Wang, Qianru ;   Moskowitz, Samuel ;   Buie, Cullen Richard

Recently we've demonstrated insulator based dielectrophoresis with strain-level discrimination of the opportunistic human pathogen Pseudomonas aeruginosa. P. aeruginosa is the chief organism involved in lung infections of patients with cystic fibrosis. A major virulence factor associated with P. aeruginosa infections is its development of antibiotic resistance. It is known that P. aeruginosa antibiotic resistance is associated with physical changes to the cell envelope. We hypothesize that these phenotypic changes also alter the electrical properties of the cell envelope. In this study we will utilize insulator based dielectrophoresis to detect subtle changes in electrical phenotype associated with antibiotic resistance. This work will enable the development of culture free diagnostics of antibiotic susceptibility.