Digital and Multiplexed Protein Quantification by Microfluidic Very Large Scale Integration

Araci, Ismail Emre ;   Robles, Michael C. ;   Quake, Stephen

We have designed and fabricated a reusable digital protein detection platform and demonstrated its usage for b-galactosidase expression analysis in E. coli and for detection of TNF-a protein. More than an order of magnitude reduction in PDMS valve dimensions allowed various critical functionalities such as multiplexing, washing, mixing and reaction at femtoliter volumes.