High Throughout, Microprinting of Tumor Spheroids for Anti-Cancer Drug Screening
We report a new approach to generating tumor spheroids using a polymeric aqueous two-phase system (ATPS). Cancer cells are confined in an aqueous phase drop, which is ?printed? into a bath of a second aqueous phase. Immiscibility of the two phases and confinement of cells to the drop facilitates formation of a viable tumor spheroid within the drop. A panel of pairs of biocompatible polymers is identified to support spheroid generation with ATPS. The technology is adapted to standard microplates and robotic liquid handling to enable high throughput drug screening and discovery of novel chemotherapeutics against 3D tumor models.