Formation of Spatially-Defined Heterogeneous ECM-Coated Surfaces Using Microfluidic Patterning with Capillary Stop Valves to Control Cell Adhesion of Stem Cells

Kaneda, Shohei ;   Nakamura, Hiroko ;   Kawada, Jiro ;   Ono, Koichi ;   Fujii, Teruo

A microfluidic device to control condition of cell adhesiveness by the surface coated with two different extracellular matrix (ECM) proteins was developed. The spatially-defined heterogeneous ECM-coated surfaces were formed in the device by using capillary action and capillary stop valves. As a demonstration, both formation of two different ECM-coated surfaces with laminin and vitronectin, and control cell adhesion of an embryoid body (EB) formed from mouse induced pluripotent stem cells (miPSCs) were performed in the single microfluidic device.