Real-Time Detection and Closed Loop Feedback of Biomolecules in Live Animals

Soh, H. Tom

A biosensor capable of continuously measuring specific molecules in the bloodstream in vivo would give clinicians a valuable window into patients? health and their response to therapeutics. Unfortunately, continuous, real-time measurement is currently only possible for a handful of targets (i.e. glucose, lactose, and oxygen) and existing platforms for continuous measurement are not generalizable for the monitoring of other analytes. In this presentation, we will discuss the development of a universal real-time biosensor technology capable of continuously tracking a wide range of circulating molecules in living animals. Our microfluidic electrochemical detector for in vivo continuous monitoring (MEDIC) requires no exogenous reagents, operates at room temperature, and can be reconfigured to measure different target molecules by exchanging probes in a modular manner. To demonstrate the system?s versatility, we will present real-time measurement of doxorubicin (a chemotherapeutic) and kanamycin (an antibiotic) in live rats with sub-minute temporal resolution. Finally, using the MEDIC sensor, we report the first closed-loop feedback control of drug concentration in live animals.