Programmable Microfluidic Digital Logic for the Autonomous Lab-on-a-Chip

Ahrar, Siavash ;   Duncan, Philip ;   Raje, Manasi ;   Hui, Elliot

We present sequential logic circuits implemented in microfluidics rather than electronics. A programmable finite state machine was constructed out of pneumatic Boolean logic gates and was employed to control liquid handling operations such as sequential washing for immunoassays and serial dilution. High-density circuitry was achieved by precision machining of plastic, enabling a 12-bit sequential counter to fit within 360 square mm of die area.