Digital Drug Dosing: Dosing in Drug Assays by Light-Defined Volumes of Hydrogels with Embedded Drug-Loaded Nanoparticles

Faralli, Adele ;   Melander, Fredrik ;   Larsen, Esben Kjær Unmack ;   Andresen, Thomas Lars ;   Larsen, Niels Bent

Polyethylene glycol (PEG)-based hydrogels are widely used for biomedical applications, including matrices for controlled drug release. We present a method for defining drug dosing in screening assays by light-activated cross-linking of PEG-diacrylate hydrogels with embedded drug-loaded liposome nanoparticles in freely definable areas of micro wells.