Optical Cell Separation from 3D Culture Environment in Photodegradable Hydrogels for Pure Culture Techniques

Tamura, Masato ;   Yanagawa, Fumiki ;   Sugiura, Shinji ;   Takagi, Toshiyuki ;   Sumaru, Kimio ;   Matsui, Hirofumi ;   Kanamori, Toshiyuki

We present a new methodology for optical cell picking in 3D culture environment. In this study, we encapsulated cells in photodegradable hydrogels by mixing cell-containing gelatin solution with crosslinker, NHS-PC-4armPEG. The minimum resolution of photodegradation was estimated around 20 ┬Ám. The encapsulated cells in the computer-controlled light irradiated area can be selectively separated and retrieved without any damages in terms of cell growth. This methodology and the photodegradable hydrogels in this study are applicable to cell separation based on the cellular morphology in 3D environment.