Multi-Scale Topographical Patterned Substrates for Promoting Differentiation of Human Neural Stem Cells

Yang, Kisuk ;   Jung, Hyunjung ;   Lee, Hak-Rae ;   Bang, Joona ;   Im, Sung Gap ;   Cho, Seung-Woo

Self-renewal and differentiation of human neural stem cells (hNSCs) can be controlled by surface topography of culture substrates. In this study, we develop a multi-scale, hierarchically patterned substrate (HPS) for promoting neuronal differentiation of hNSCs. HPS significantly enhanced focal adhesion formation and neuronal differentiation of hNSCs. Interestingly, hNSCs differentiated on the HPS exhibited electrophysiological characteristics of functional neuron. HPS can facilitate lineage specification of hNSCs towards functional neuron.