Comparative Transfection of DNA by AuPAMAM Nanoparticles

Figueroa, Elizabeth ;   Yan, Stepehn ;   Chamberlain-Simon, Nicolette ;   Chen, Allen ;   Drezek, Rebekah

In this study, we decipher differences in gene delivery by cationic gold-polyamidoamine nanoparticle (AuPAMAM) vectors in easy- and difficult-to-transfect cell lines. The aim of the work is to elucidate the contribution of cellular uptake, endosomal escape, and cytoplasmic transport to the overall gene delivery process. The transport of plasmid DNA by PEI and AuPAMAM was compared in SKBR3 breast cancer cells, B16-F10 melanoma cells, JAWS II murine dendritic cells and CT26 colon carcinoma cells using flow cytometry, cellular TEM and confocal fluorescence microscopy to shed light on the mechanistic hurdles that make specific cell lines hard to transfect.