Encapusulation of Natural Synthesized Novel Nutraceutical Biomolecules

Kermasha, Selim ;   Aziz, Sarya

There has been growing evidence supporting the nutritional benefits of long-chain n-3-polyunsaturated fatty acids (ω-3-PUFAs), in particular eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), for human health. In addition to their biological activities, phenolic acids (PAs) are commonly known as natural antioxidants, where their antioxidant capacity is due to their ability of scavenging the free radicals. However, the use of PAs in fat and oil systems is limited by their hydrophilic nature. Research work in our laboratory succeeded in the production of novel biomolecules, phenolic lipids (PLs), by a biotechnological process involving the esterification of a phenolic acid model, 3,4 dihydroxyphenylacetic acid (DHPA), with a selected marine oil rich in phospholipids, krill oil (KO). However, it was important to ensure the preservation and the delivery of these novel nutraceuticals by their encapsulation. The optimization of the encapsulation of phenolic lipids (PLs) was carried out by the development of a process to yield gelatin-gum arabic multinuclear microcapsules of krill oil (KO), via complex coacervation. In comparison to the unmodified KO, the presence of PLs and DHPA had a major effect on the size, morphology of the capsules. Initial screening experiments were performed to identify the parameters that have the most significant effectson the encapsulation efficiency (EE) of PLs. The presence of PLs resulted in the formation of capsules at the emulsification