Needle Microelectrodes for Electromyography Recording from Zebrafish Larvae

Cho, Sung-Joon ;   Nam, Tae-Seung ;   Choi, Seok-Yong ;   Kim, Myoung-Kyu ;   Kim, Sohee

Electromyography (EMG) is the most effective tool in diagnosing muscular diseases, and it is an essential tool in developing treatment agents for such diseases. However, because of the pain and discomfort experienced during needle EMG test procedure, the development of treatment agents for such diseases is hardly feasible. Therefore, measuring EMG signals on smaller vertebrates such as zebrafish larvae will greatly contribute in developing treatments for incurable muscular diseases. In this study, we introduce an EMG micro-needle electrode that is designed for small vertebrates such as zebrafish larvae. Firstly, the design and fabrication of the micro-needle electrodes are explained followed by the recorded EMG signals from 30 days post-fertilization (dpf) zebrafish larvae.