Microfluidic Devices for Oxygen Control

Eddington, David

We have developed a suite of tools to control oxygen concentrations in mammalian cell cultures and tissues. These devices leverage the innate gas permeability of PDMS and rapid diffusion at the microscale to set up experimental conditions either not possible at the macroscale or exceedingly difficult. This paper will describe these devices and their applications including a device to oxygenate a wound model, a brain slice preparation, and an endothelial cell culture.