Real Time Impedance Sensing of Intermediate Stages of Mitosis of a Single Cell

Kaji, Hirokazu ;   Ghenim, Lamya ;   Nagamine, Kuniaki ;   Porte, Stephanie ;   Haguet, Vincent ;   Gidrol, Xavier ;   Nishizawa, Matsuhiko

We have constructed a sensitive tool to monitor electrically changes of individual cells by combining electrochemical-based surface modification and positive dielectrophoresis with micrometric electrodes. Electrical cell-substrate impedance sensing coupled with optical observation has defined an electrical characterization for intermediate stages of mitosis for a single HeLa cell. Monitoring the impedance changes associated with the cell cycle might define differences between a single cancerous or healthy cell.