Advance Diagnostic and Sample Preparation Platform for Biosurveillance

Jebrail, Mais ;   Sinha, Anupma ;   Renzi, Ron ;   Bartsch, Micheal ;   Van De Vreugde, Jim ;   Branda, Steven ;   Patel, Kamlesh

We report on our work to develop a forward-deployed sample preparation and diagnostic platform to safely and consistently process infected clinical samples in the field for analysis as part of a comprehensive biosurveillance strategy. Small-volume blood samples are processed on a digital microfluidic hub platform with integrated modules to 1) extract and process RNA and 2) stabilize and format RNA into a cDNA intermediate for either real-time multiplex PCR analysis or lab-based next generation sequencing.