Reading Out Single-Molecule Digital RNA and DNA Isothermal Amplification Visually and with Unmodified Cell Phones

Rodriguez Manzano, Jesus ;   Karymov, Mikhail ;   Begolo, Stefano ;   Selck, David ;   Zhukov, Dmitriy ;   Jue, Erik ;   Ismagilov, Rustem

We developed a methodology for visual digital readout that can be used to detect and quantify single nucleic acid molecules using a microfluidic chip and an unmodified cellphone, suitable for diagnostic tests in point-of-care.