Point-of-Care Test by Gold Nanoparticle Aggregation: Effects of Nanoparticle Concentration and Size

Revuru, Naga Aravind ;   Kang, Peiyuan ;   Qin, Zhenpeng

Point-of-Care (POC) diagnosis of infectious diseases continues to be in urgent need and can lead to early diagnosis and treatment. The gold nanoparticle-based aggregation assay is a simple, fast, and colorimetric detection method, suitable for POC environments. In this work, we characterize an important aspect of the assay including the nanoparticle probe concentration and size. Preliminary results suggest that higher GNP probe concentration shifts the assay dynamic range to be less sensitive. Our work presents guidelines to design this nanoparticle assay to meet sensitivity needs.