Segmentation of T Cells in Fluorescence Microscopy

Antoni, Sven-Thomas ;   Dabrowski, Adam ;   Schetelig, Daniel ;   Diercks, Björn-Philipp ;   Fliegert, Ralf ;   Werner, René ;   Wolf, Insa M. A. ;   Guse, Andreas H. ;   Schlaefer, Alexander

In the adaptive immune system Calcium (Ca2+) is acting as a fundamental on-switch with. Fluorescence microscopy is used to study the underlying mechanisms. However, liv- ing cells introduce motion, hence a precise segmentation is need- ed for subsequent analysis of (sub-)cellular Ca2+ activity. We extend a segmentation algorithm and evaluate its performance. Results indicate that robust segmentation of the cells is possible.